We are our DNA!

Held in our cell nuclei, DNA is a long chain of tightly wound-up coded instructions which cells unravel, read and use to make proteins or polypeptides.

When cells make a certain protein, they read DNA in chunks called genes that code for a specific sequence of amino acids.

 

Like proteins, DNA is also a polymer but of repeating nucleotides - units made of a phosphate group on a sugar attached to a nitrogenous base.

Nucleotides link together to make the double helix structure of DNA.

 

There are four different bases, A, T, C, G, and they’re very specific about what they pair with:

Adenine with Thymine and Cytosine with Guanine

 

These groups are complementary base pairs, and they are joined by weak hydrogen bonds, linking the two double helix strands in the middle!

 

 

So far, we have only said that DNA codes for proteins. This is not entirely true, because there are two types!

Coding DNA are the genes  that the cells read and make proteins from.

Non-coding DNA are like on-off switches for coding DNA - they control gene expression by telling the cell to use or ignore certain genes.

 

 

So, to make a protein there are two stages: transcription of DNA (in the nucleus) and translation  (in the cytoplasm):

 

Transcription:

The cell reads the genes to find the amino acid order - but DNA is too big to get out of the nucleus to build the protein, so it uses a messenger instead!

 An enzyme temporarily breaks the weak hydrogen bonds and unzips the double helix. It then uses one side as a template to make a copy of the genes there, with this new copy being called mRNA.

 mRNA travels out into the cytoplasm and carries the code to the site of protein synthesis - an organelle called a ribosome.

 

 

Translation:

As the ribosome inspects the mRNA, it reads the nucleotide bases in groups of three called codons, with one codon coding for one specific amino acid.

 Then another helper called transfer or tRNA brings 100s to1000s of specific amino acids to the ribosome in the sequence that the genes state, which are then linked to make the initial polypeptide.

After protein synthesis, the polypeptide then folds into a specific 3D structure.

 

 

But change happens, and when the order of bases changes in our genes, we call this a mutation.

Mutations can change proteins through our DNA, but usually their function is preserved and little difference is seen in our phenotypes.

But occasionally, if enough of the coding or non-coding DNA is changed, the protein’s shape can be altered so much that it can’t do its job, like an enzyme without a working active site.

 

Now on to some questions!