Loading please wait

The smart way to improve grades

Comprehensive & curriculum aligned

Try an activity or get started for free

Apply Knowledge of Diffusion

In this worksheet, students will apply knowledge of factors that impact diffusion.

'Apply Knowledge of Diffusion' worksheet

Key stage:  KS 4

Year:  GCSE

GCSE Subjects:   Component 1: Concepts in Biology: Single Subject, Component 1: Concepts in Biology: Combined Science,

GCSE Boards:   Eduqas

Curriculum topic:   Transport Systems

Curriculum subtopic:   Transport in Cells

Difficulty level:  

Worksheet Overview

Image of a pair of socks



Why can you smell someone's stinky PE socks from all the way across the classroom? Yuk! Normally, it's because sweat and other molecules are moving away from the socks and spread out in the air. This is called diffusion. 


Diffusion is the spreading out of the particles of any substance in solution, or particles of a gas, resulting in movement from an area of higher concentration (where there's more particles in a certain solution or area) to an area of lower concentration (where there are fewer particles).  


Image of particles diffusing


Living organisms need different substances to be able to survive and function. These substances need to be transported into and out of their cells through diffusion (as well as osmosis and active transport - you will learn more about these later!). During diffusion, molecules move from an area of high concentration to an area of low concentration. They are said to move down a concentration gradient. Particles diffuse until they are evenly spaced apart. Diffusion is a passive process, which means that no energy is needed and it happens naturally. 


The surface area to volume ratio of an organism means how much area an organism has in comparison to its volume. As organisms get larger, their surface area to volume ratio gets smaller - for example, an elephant has a smaller surface area to volume ratio than a mouse! Larger organisms tend to have special adaptations to overcome their smaller surface area to volume ratio - read on to find out more!


Image of gas exchange in alveolus



Diffusion in organisms

The cell membrane has an important job to do. It is selective. This means it won't just let any random particle through - it can monitor which particle is allowed through it and also how many particles. Pretty clever right?!​

In multicellular organisms, surfaces and organ systems are specialised for exchanging materials. This is to allow sufficient molecules to be transported into and out of cells for the organism’s needs. Diffusion is the main way that substances move over short distances in organisms.

Breathing involves exchanging gases in the lungs -  this requires diffusion. When you breathe in, oxygen in the inhaled air diffuses through the tiny alveoli (air sacs) in your lungs into your bloodstream. The oxygen is transported around your body. Carbon dioxide is the waste gas produced by respiration. Carbon dioxide diffuses from cells into the bloodstream and is exhaled by the lungs. 

The alveoli have a few adaptations that make gas exchange very efficient. They are only one cell thick, making them very thin, allowing gases to pass through easily and quickly. They also have a large combined surface area, allowing large amounts of gases to be exchanged with each breath.


Image of villi in intestine


Another example of diffusion is in the small intestine. Digested food is broken down into small molecules such as glucose and amino acids. These important molecules need to be transported around the body in the blood. The small intestine is lined with many finger-like projections called villi. The molecules diffuse through the villi of the small intestine into the blood to be transported around the body. The villi are adapted for this by being very thin and having finger-like projections that increase the surface area. Villi also have partially permeable membranes. This means they have pores that allow smaller molecules through, but not larger molecules.  This can be demonstrated in the ​science lab using something called a Visking tube.  The Visking tube acts as a model of a cell membrane. You can see a diagram of a Visking tube below: 


Visking Tubing


The Visking tube can mimic the action of the gut by allowing small molecules, such as glucose,  to diffuse out, while larger molecules can't. There are other similarities between Visking tubing and a real gut -  for example, ​the food in the Visking tube is a mixture of large and small molecules, much like the digested food in our intestines. This is why the Visking tube is used as a model of the intestines. There are some limitations, however, as with all models. For example, the Visking tubing membrane is smooth, whereas the lining of the gut is folded, with a large surface area. Also, the intestines are surrounded by blood vessels ready to transport substances around the body.


Image of plant photosynthesising Image of leaf anatomy


Diffusion also occurs in plants. Plants take in carbon dioxide for photosynthesis and produce oxygen. These gases enter and leave the plant through the process of diffusion. The structure of the leaf is well adapted for gas exchange. There are tiny pores, called stomata, in the surface of the leaf. There are usually more stomata on the underside of a leaf than on the topside. The stomata allow carbon dioxide to enter the leaf for photosynthesis. Molecules of carbon dioxide diffuse from a region of higher concentration (the atmosphere) to a lower concentrated area (leaf). The stomata also allow oxygen to diffuse out.  


Factors affecting diffusion

Different factors can affect diffusion and how quickly it happens. Some of these factors are:

The difference in concentrations (concentration gradient) - having a large difference in concentrations means that diffusion can occur at a quicker rate, as particles will naturally move from a high to low concentration. 
The temperature - the higher the temperature, the more energy the particles will have to move and spread out.
The surface area of the membrane - the larger the surface area, the faster the rate of diffusion. This is because more particles can pass through the membrane since there is more area, as in the alveoli in the lungs.
thin membrane - to provide a short diffusion path.

In the following activity, you will apply your knowledge of factors that affect the process of diffusion.


What is EdPlace?

We're your National Curriculum aligned online education content provider helping each child succeed in English, maths and science from year 1 to GCSE. With an EdPlace account you’ll be able to track and measure progress, helping each child achieve their best. We build confidence and attainment by personalising each child’s learning at a level that suits them.

Get started

Try an activity or get started for free

  • National Tutoring Awards 2023 Shortlisted / Parents
    National Tutoring Awards 2023 Shortlisted
  • Private-Tutoring-WINNER-EducationInvestor-Awards / Parents
    Winner - Private Tutoring
  • Bett Awards Finalist / Parents
  • Winner - Best for Home Learning / Parents
    Winner - Best for Home Learning / Parents