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Explore Factors that Determine Transpiration Rates

Worksheet Overview

Did you know that plants only use about 5 - 6% of all the water they absorb for photosynthesis? So what happens to all that water?  Let's find out more below.

Image of photosynthesis


Plants can’t help but lose water continually to the air. This is called transpiration. 

Water is constantly lost from the leaves of a plant through pores called stomata. When a plant opens its stomata to allow carbon dioxide in for photosynthesis, water will evaporate and diffuse out of the stomata. More water is drawn up from the stem and the roots to replace the lost water. This is because a concentration gradient exists - there's more water in the roots than the leaves. Water is also naturally cohesive. This means that water tends to 'stick' together, which helps to pull it up through the xylem vessels.

Water moves by osmosis from a high concentration in the roots to a lower one in the leaves. As water moves from the roots to the leaves, more water is drawn up from the soil into the root hair cells. This occurs because the concentration of water in the soil is greater than in the roots - the soil has a higher water potential, so water moves by osmosis into the root hair cells. 

This process is known as the transpiration stream.

Although transpiration is inevitable, it's also quite useful! It helps the plant remain cool and allows minerals to be drawn up the plant along with the water. It also gives plants structure and support.


Factors affecting transpiration

Transpiration is affected by many factors:

Temperature - increasing the temperature makes transpiration happen faster - the plant loses more water from its leaves. This is because water evaporates at a faster rate when it's warmer, so there'll be a greater concentration gradient. Water will be drawn up from the roots to replace the 'missing' water in the leaves, moving from a high concentration of water (in the roots) to a lower concentration in the leaves.

Humidity - if it's really humid, it means there's a lot of moisture in the air. The concentration of water outside the plant is higher than inside the plant, so the plant doesn't transpire as much and doesn't lose as much water.  

Wind - if it's really windy, water vapour is blown away from the leaf.  The concentration of water outside the plant is lower than inside the plant, causing the plant to lose more water from its leaves.

Light intensity - if its really sunny, the stomata will open wide to let in more carbon dioxide for photosynthesis -  at the same time, the plant will lose water.


Plant adaptations

Plants have adaptations that allow it to do a particular job.


Root Hair Cell


Image of root hair cell


Root hair cells are specialised cells found at the roots of a plant (see image above). These cells are long and thin, making them useful to manoeuvre between soil particles in search of water. The large surface area of the root hair cell allows for a greater chance of contact with water.​ Root hair cells contain lots of mitochondria, which provides the cell with energy. This energy is essential for active transport, which is needed to take in minerals from the soil.




Image of leaf anatomy



Another adaptation of the plant is found in the leaf​. The lower epidermis layer contains the stomata (stoma for one pore). These stomata allow gases in and out of the underside of the leaf. The stomata are found between guard cells which open or close the stomata depending on how turgid (full of water and swollen - stomata open) or flaccid (lacking water and shrunken - stomata closed) the guard cells are. The stomata will open to allow in carbon dioxide for photosynthesis during the day, but will close during the night when there's no sunlight. 


 Image of xylem and phloem vessels



Water moves through xylem vessels.  A xylem vessel is a continuous hollow tube that transports water and minerals in one direction from a plant's roots to the plant's leaves, via the stem. This allows water to travel uninterruptedly up the stem to the leaves. Xylem vessels are made of lignin that strengthen the walls. This helps to support the plant.



Another transport vessel the plant has is called the phloem. The phloem vessels move sucrose (a form of sugar) that the plant has made by photosynthesis to where it's needed (for example in growing parts of the plant and for storage). Food travels up and down the stem using energy. The cells that make up the phloem are living, and include cells called sieve tubes and companion cells which help to transport substances in the phloem. This is known as translocation.


Measuring transpiration



Image of a Potometer  Image of a potometer


Scientists have been able to work out how to measure transpiration in the science lab. A potometer (shown above) is used to measure how much water is being taken up by the plant and the rate at which it does so.

Here's how to use a simple potometer:


1) The potometer is filled with water.

2) A leafy plant is connected to it underwater through a rubber tube at one end of the potometer.

3) A bubble of air passes up the potometer when held upright and then placed back into the water.

4) The bubble is observed and the distance travelled by the bubble over a set period of time is measured.

5) The faster the bubble moves, the greater the rate of water uptake and so the greater the rate of transpiration.

The conditions can be varied depending on what you want to investigate -  for example, the effect of changing temperature, humidity,  light intensity or wind speed (for example, with a fan).

In the following activity, you will discuss the factors affecting transpiration.

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